LOCUS       sequence-60427-e       10933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-EF1a-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1873..2106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        2784..3967
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3015..3959
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3916..3936
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3987..4700
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4790..5378
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4843..4863)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5261..5272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6286..6510
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6643..6662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7683..7700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7854..8442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7934..7953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8613..9404,9405..9473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9236..9255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9474..9578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9646..9664
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9715..9737)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9823..9961
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9876..9895
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
      901 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
      961 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     1021 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     1081 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     1141 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     1201 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     1261 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     1321 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     1381 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     1441 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     1501 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     1561 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     1621 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     1681 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     1741 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1921 cgcacggtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     1981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2041 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2161 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     2221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     2341 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2461 acaaattact aaaaattcna aaattttcgg gtttattaca gggacagcag agatccactt
     2521 tgggcggccg ctttggagct aagccagcaa tggtagaggg aagattctgc acgtcccttc
     2581 caggcggcct ccccgtcacc acccccccca acccgccccg accggagctg agagtaattc
     2641 atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa actcccacta
     2701 acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct cgtcatcact
     2761 gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     2821 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     2881 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     2941 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3001 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3061 atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat
     3121 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3181 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     3241 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     3301 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     3361 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     3421 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     3481 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     3541 gcggcaacag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3601 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3661 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3721 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     3781 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3841 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3901 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3961 tgtcgtggcg gccgctagcc gccaccatga gcaagggcga ggaactgttc actggcgtgg
     4021 tcccaattct cgtggaactg gatggcgatg tgaatgggca caaattttct gtcagcggag
     4081 agggtgaagg tgatgccaca tacggaaagc tcaccctgaa attcatctgc accactggaa
     4141 agctccctgt gccatggcca acactggtca ctaccctgac ctatggcgtg cagtgctttt
     4201 ccagataccc agaccatatg aagcagcatg actttttcaa gagcgccatg cccgagggct
     4261 atgtgcagga gagaaccatc tttttcaaag atgacgggaa ctacaagacc cgcgctgaag
     4321 tcaagttcga aggtgacacc ctggtgaata gaatcgagct gaagggcatt gactttaagg
     4381 aggatggaaa cattctcggc cacaagctgg aatacaacta taactcccac aatgtgtaca
     4441 tcatggccga caagcaaaag aatggcatca aggtcaactt caagatcaga cacaacattg
     4501 aggatggatc cgtgcagctg gccgaccatt atcaacagaa cactccaatc ggcgacggcc
     4561 ctgtgctcct cccagacaac cattacctgt ccacccagtc tgccctgtct aaagatccca
     4621 acgaaaagag agaccacatg gtcctgctgg agtttgtgac cgctgctggg atcacacatg
     4681 gcatggacga gctgtacaag gaattcccgg gtcgactctg actgactgag gcctctcgta
     4741 cgacgcgtgc tagcatgcct aggtcgaccc gggatccgcg gactagtata atcaacctct
     4801 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4861 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4921 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4981 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5041 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5101 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5161 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     5221 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     5281 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     5341 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gaccactagt
     5401 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
     5461 taaccgggcg agggggatct cgactctaga agcttggtac ctttaagacc aatgacttac
     5521 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5581 cactcccagt ttcgagttgg cgcgcctggg agctcacggg gacagccccc cccaaagccc
     5641 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     5701 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     5761 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     5821 cagacacctg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     5881 gcgcgcctgg gagctcacgg ggacagcccc ccccaaagcc cccagggatg taattacgtc
     5941 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     6001 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     6061 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg
     6121 gggaaaaagc tttaggctga aagagagatt tagaatgaca ggcgcgccaa ctcgaattca
     6181 ttgcagactt tcactgaaac gggtctctct ggttagacca gatctgagcc tgggagctct
     6241 ctggctaact agggaaccca ctgcttaagc ctcaataaag ctcgactgtg ccttctagtt
     6301 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6361 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6421 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6481 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6541 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6661 cttcctttct cgccacgttc gccggctgtg cgggagaacg gagttctatt atgactcaaa
     6721 tcagtctccc caagcattcg gggatcagag tttttaagga taacttagtg tgtagggggc
     6781 cagtgagttg gagatgaaag cgtagggagt cgaaggtgtc cttttgcgcc gagtcagttc
     6841 ctgggtgggg gccacaagat cggatgagcc agtttatcaa tccgggggtg ccagctgatc
     6901 catggagtgc agggtctgca aaatatctca agcactgatt gatcttaggt tttacaatag
     6961 tgatgttacc ccaggaacaa tttggggaag gtcagaatct tgtagcctgt agctgcatga
     7021 ctcctaaacc ataatttctt ttttgttttt ttttttttat ttttgagaca gggtctcact
     7081 ctgtcaccta ggctggagtg cagtggtgca atcacagctc actgcagcct caacgtcgta
     7141 agctcaagcg atcctcccac ctcagcctgc ctggtagctg agactacaag cgacgcccca
     7201 gttaattttt gtatttttgg tagaggcagc gttttgccgt gtggccctgg ctggtctcga
     7261 actcctgggc tcaagtgatc cagcctcagc ctcccaaagt gctgggacaa ccggggccag
     7321 tcactgcacc tggccctaaa ccataatttc taatcttttg gctaatttgt tagtcctaca
     7381 aaggcagtct agtccccagg caaaaagggg gtttgtttcg ggaaagggct gttactgtct
     7441 ttgtttcaaa ctataaacta agttcctcct aaacttagtt cggcctacac ccaggaatga
     7501 acaaggagag cttggaggtt agaagcacga tggaattggt taggtcagat ctctttcact
     7561 gtctgagtta taattttgca atggtggttc aaagactgcc cgcttctgac accagtcgct
     7621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggcg ctcttccgct
     7681 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7741 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8041 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8281 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9601 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9661 aggccctttc gtcttcaaga actgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     9721 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9781 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     9841 acgtagcgat agcggagtgt actggcttaa ctatgcggca tcagagcaga ttgtactgag
     9901 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9961 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    10021 gctattacgc cagcgcgggg aggcagagat tgcagtaagc tgagatcgca gcactgcact
    10081 ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat
    10141 attccaatct tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct
    10201 tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct
    10261 tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa aggtaaaaaa
    10321 taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat ggagatacag
    10381 ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag cgcctctctc
    10441 caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc tgtgctgcca
    10501 accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag tgtgccctga
    10561 ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta gtttcagtta
    10621 cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact gcgagagaaa
    10681 gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag cctcacaggg
    10741 ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc acagtgcacc
    10801 cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct aacttgtaag
    10861 ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg gttcagtact
    10921 atcgcatttc agg
//
