LOCUS       sequence-60429-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TimeSTAMP method for tagging and visualization of newly synthesized 
            proteins. TimeSTAMPt cassette used for highly expressed proteins or 
            proteins with faster ongoing synthesis rates.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lin MZ, Tsien RY
  TITLE     TimeSTAMP tagging of newly synthesized proteins.
  JOURNAL   Curr Protoc Protein Sci. 2010 Feb;Chapter 26:Unit 26.5. doi: 
            10.1002/0471140864.ps2605s59.
  PUBMED    20155731
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Protein Sci."; date: "2010-02"; volume: "Chapter 26:Unit 26.5. doi";
            pages: " 10.1002/0471140864.ps2605s59"
COMMENT     Sequence Label: pCS6-mPSD95a-TSt-HA
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             815..841
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gaagcaaatg gggtcgacgc tctaccagga gttcgatgag atggaagagt gctctcagca
       61 cttacccggc gccggcagta gtggcgatat catggattac aaggatgacg acgataaggg
      121 ctcttccggg acaggctccg gatccggcac tagtgcgccc atcacggcgt acgcccagca
      181 gacgagaggc ctcctagggt gtataatcac cagcctgact ggccgggaca aaaaccaagt
      241 ggagggtgag gtccagatcg tgtcaactgc tacccaaacc ttcctggcaa cgtgcatcaa
      301 tggggtatgc tggactgtct accacggggc cggaacgagg accatcgcat cacccaaggg
      361 tcctgtcatc cagatgtata ccaatgtgga ccaagacctt gtgggctggc ccgctcctca
      421 aggttcccgc tcattgacac cctgtacctg cggctcctcg gacctttacc tggtcacgag
      481 gcacgccgat gtcattcccg tgcgccggcg aggtgatagc aggggtagcc tgctttcgcc
      541 ccggcccatt tcctacttga aaggctcctc ggggggtccg ctgttgtgcc ccgcgggaca
      601 cgccgtgggc ctattcaggg ccgcggtgtg cacccgtgga gtggctaaag cggtggactt
      661 tatccctgtg gagaacctag agacaaccat gagatccccg gtgttcacgg acaactcctc
      721 tccaccagca gtcaccctga cgcacccaat caccaaaatc gatagggagg ttctctacca
      781 ggagttcgat gagatggaag agtgctctca gcactatccc tacgatgtgc ccgattacgc
      841 tggcgcgcct
//
