LOCUS       sequence-60433-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Ouyang X, Winbow VM, Patel LS, Burr GS, Mitchell CK, O'Brien J
  TITLE     Protein kinase A mediates regulation of gap junctions containing 
            connexin35 through a complex pathway.
  JOURNAL   Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11.
  PUBMED    15857663
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain Res 
            Mol Brain Res."; date: "2005-04-27"; volume: "135(1-2)"; pages: 
            "1-11"
COMMENT     Sequence Label: PCx35CT-S298ter
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..41)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(270..281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(312..356)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(372..389)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(399..416)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(464..486)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 taggtattaa tcaattagtg gtggtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtatgtgt gtgtttgtta cacataagca gagtcactct actgagtacg gccgaaatta
      121 ggcacactca tcctcggcaa gtccttattt ctgatctcat aaatggactt ccgccgagcc
      181 tgcactcctc tcacggccgt tttgattttc ctccagccca ggtgattgag ttcttctaga
      241 attcggatcc ccgatatcgc catggacccg cgtccctcaa taccggagcc accaccggta
      301 cccagatctg ggctgtccat gtgctggcgt tcgaatttag cagcagcggt ttctttcata
      361 ccaattgcag tactaccgcg tggcaccaga cccgcggagt gatggtgatg gtgatgacca
      421 gaaccactag ttgaaccatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc
      481 cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg
      541 atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg
      601 tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg ggttacatga
      661 tcaccattta aatatgtttt atgacataaa cgatcttcga acattttcag catttcaggt
      721 agcttgctaa gaaaatcaac tttgagagtt tcaaagtctt tactatatgc aattctcgaa
      781 acaccgtatc taatatccaa aaccgctcct tcaagcattg aaatctctgc acgctctttt
      841 ggac
//
