LOCUS       sequence-60435-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Ouyang X, Winbow VM, Patel LS, Burr GS, Mitchell CK, O'Brien J
  TITLE     Protein kinase A mediates regulation of gap junctions containing 
            connexin35 through a complex pathway.
  JOURNAL   Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11.
  PUBMED    15857663
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain Res 
            Mol Brain Res."; date: "2005-04-27"; volume: "135(1-2)"; pages: 
            "1-11"
COMMENT     Sequence Label: PCx35CT-S276A, S298ter
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..46)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(275..286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(317..361)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(377..394)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(404..421)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(469..491)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cagcctaggt attaatcaat tagtggtggt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgta tgtgtgtgtt tgttacacat aagcagagtc actctactga gtacggccga
      121 aattaggcac actcatcctc ggcaagtcct tatttctgat ctcataaatg gccttccgcc
      181 gagcctgcac tcctctcacg gccgttttga ttttcctcca gcccaggtga ttgagttctt
      241 ctagaattcg gatccccgat atcgccatgg acccgcgtcc ctcaataccg gagccaccac
      301 cggtacccag atctgggctg tccatgtgct ggcgttcgaa tttagcagca gcggtttctt
      361 tcataccaat tgcagtacta ccgcgtggca ccagacccgc ggagtgatgg tgatggtgat
      421 gaccagaacc actagttgaa ccatccgatt ttggaggatg gtcgccacca ccaaacgtgg
      481 cttgccagcc ctgcaaaggc catgctatat acttgctgga tttcaagtac ttatcaattt
      541 gtgggatagc ttcaatacgt tttttaaaac aaactaattt tgggaacgca tccaggcaca
      601 ttgggtccat gtataaaaca acatcaagag cgtcatacaa catgaagtca ggatgggtta
      661 catgatcacc atttaaatat gttttatgac ataaacgatc ttcgaacatt ttcagcattt
      721 caggtagctt gctaagaaaa tcaactttga gagtttcaaa gtctttacta tatgcaattc
      781 tcgaaacacc gtatctaata tccaaaaccg ctccttcaag cattgaaatc tctgcacgct
      841 cttttggaca accacccaac
//
