LOCUS       sequence-60436-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(8..26)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    399..446
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gagaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
       61 tagtatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag
      121 gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca
      181 tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc
      241 atataagtac gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata
      301 tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc
      361 gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc
      421 tattctctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga
      481 cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat
      541 accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc
      601 ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac
      661 attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt
      721 catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag
      781 acaaaataga agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac
      841 tttctgttca caaagtatgc gcaatccaca tc
//
