LOCUS       sequence-60437-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-CGAC
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(621..639)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(622..639)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(657..673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(670..692)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    681..697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(705..711,712..729,730..735))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    750..771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(888..905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttata tagatgccgt cctagcgcgt ctcctgaccc cagagcaggt
      121 cgtggcgatc gcaagccacg acggaggaaa gcaagccttg gaaacagtac agaggctgtt
      181 gcctgtgctg tgccaagcgc acggactcac cccagagcag gtcgtggcaa tcgcgagcaa
      241 taacggcgga aaacaggctt tggaaacggt gcagaggctc cttccagtgc tgtgccaagc
      301 gcacggcctc accccagagc aggtcgtggc aatcgcctcc aacattggcg ggaaacaggc
      361 actcgagact gtccagcgcc tgcttcccgt gctgtgccaa gcgcacggat taaccccaga
      421 gcaggtcgtg gcgatcgcaa gccacgacgg aggaaagcaa gccttggaaa cagtacagag
      481 gctgttgcct gtgctgtgcc aagcgcacgg cttaagagac gtgggcacag gaaagatact
      541 taagggcgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc
      601 ttgatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
      661 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      721 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      781 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      841 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      961 cggttatcca cagaat
//
