LOCUS       sequence-60438-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    397..444
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aagggcctcg tggatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
       61 gtatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga
      121 gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg
      181 gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat
      241 ataagtacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta tatatatata
      301 caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt
      361 tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc gaagttccta
      421 ttctctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg
      481 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac
      541 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct
      601 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat
      661 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca
      721 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac
      781 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt
      841 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg gatgcc
//
