LOCUS       sequence-60446-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-HA
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    391..438
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagtatga
       61 tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca
      121 gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg
      181 gataatatca caggaggtac tagactacct ttcatcctac ataaatagac gcatataagt
      241 acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca
      301 acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt
      361 ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct
      421 agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa gacgcacttt
      481 caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga ataccgcttc
      541 cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa
      601 cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatttttta
      661 tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta ttcatagagt
      721 gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag agacnaaata
      781 gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc actttctgtt
      841 cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct ttatctt
//
