LOCUS       sequence-60456-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ultrasensitive protein calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, 
            Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, 
            Kim DS
  TITLE     Ultrasensitive fluorescent proteins for imaging neuronal activity.
  JOURNAL   Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.
  PUBMED    23868258
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-07-18- 18"; volume: "499"; issue: "7458"; 
            pages: "295-300"
COMMENT     Sequence Label: pGP-CMV-GCaMP6m
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      241 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
      481 tgccggacca actgactgaa gagcagatcg cagaatttaa agaggctttc tccctatttg
      541 acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctgg
      601 ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg
      661 acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaggg agctacaggg
      721 acacggaaga agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca
      781 tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag
      841 aggttgatga aatgatcagg gaagcagaca tcgatgggga tggtcaggta aactacgaag
      901 agtttgtaca aatgatgaca gcgaagtgag cggccgcgac tctagatcat aatcagccat
      961 accacatttg ta
//
