LOCUS       sequence-60464-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength GCaMP3-based calcium indicator with improved 
            response. Please also see the GCaMP6s/m/f indicators..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Akerboom J, Chen TW, Wardill TJ, Tian L, Marvin JS, Mutlu S, 
            Calderon NC, Esposti F, Borghuis BG, Sun XR, Gordus A, Orger MB, 
            Portugues R, Engert F, Macklin JJ, Filosa A, Aggarwal A, Kerr RA, 
            Takagi R, Kracun S, Shigetomi E, Khakh BS, Baier H, Lagnado L, Wang 
            SS, Bargmann CI, Kimmel BE, Jayaraman V, Svoboda K, Kim DS, 
            Schreiter ER, Looger LL
  TITLE     Optimization of a GCaMP calcium indicator for neural activity 
            imaging.
  JOURNAL   J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 
            10.1523/JNEUROSCI.2601-12.2012.
  PUBMED    23035093
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2601-12"; journalName: "J Neurosci"; date: 
            "2012-10-3- 3"; volume: "32"; issue: "40"; pages: "13819-40"
COMMENT     Sequence Label: pCMV-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             486..929
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      241 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc
      481 tgccggacca actgactgaa gagcagatcg cagaatttaa agaggctttc tccctatttg
      541 acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctgg
      601 ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg
      661 acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacacag
      721 acagtgaaga agaaattaga gaagcgttcc gtgtgtttga taaggatggc aatggctaca
      781 tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag
      841 aggttgatga aatgatcagg gaagcagaca tcgatgggga tggtcaggta aactacgaag
      901 agtttgtaca aatgatgaca gcgaagtgag cggccgcgac tctagatcat aatcagccat
      961 accacatttg tagaggtttt acttgcttta aa
//
