LOCUS       sequence-60469-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Laufer E, Dahn R, Orozco OE, Yeo CY, Pisenti J, Henrique D, Abbott 
            UK, Fallon JF, Tabin C
  TITLE     Expression of Radical fringe in limb-bud ectoderm regulates apical 
            ectodermal ridge formation.
  JOURNAL   Nature. 1997 Mar 27. 386(6623):366-73.
  PUBMED    9121552
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1997 Mar 27. 386(6623):366-73."
COMMENT     Sequence Label: RCAS (B) flag Lunatic Fringe (CT#471)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(235..496)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             complement(656..679)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcctgtggat cccctccgac ggtactcagc ttctgcctcc ctaagccgca gccccctcca
       61 ccagggtcat cgaactcgcc tcccgataga cgagacggat ggagacagga tcgccacgcc
      121 gtctgtggcc gaccactatt ccctaactat cacgtcgggg tcaccaaatg aagccttctg
      181 cttcatgcag gtgctcgtag tcgtcaggga atcaacggtc cggccatcaa cccaggtgca
      241 caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg agctaggcac ttaaatacaa
      301 tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacccg tctgttgcct
      361 tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt
      421 ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagagtat
      481 tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat cctgagggga
      541 ctcctaaccg cgtacaaccg aagccccgct tttcgcctaa acacacccta gtcccctcag
      601 atacgcgtat atctggcccg tacatcgcat cgattctaga ctgcagttag gatcccttgt
      661 catcgtcgtc cttgtagtcg ctagcgtaca ccacgttgga agggcaccac ggcgtgtcgg
      721 ggtacagcag gcagtgcaca gagcgaaacc tggatgggtc ctcctccacg gagaaggctc
      781 ccttcatgtg gatggagttg cgcttgtttt caaacatgcc atagcttagt gtcacctgct
      841 tgtggatctc tgtcttgggc acctggtgaa ggtt
//
