LOCUS       sequence-60472-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Mali P, Aach J, Lee JH, Levner D, Nip L, Church GM
  TITLE     Barcoding cells using cell-surface programmable DNA-binding domains.
  JOURNAL   Nat Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407.
  PUBMED    23503053
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407."
COMMENT     Sequence Label: sZF13
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     146..205
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             209..235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             257..280
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             590..619
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             623..769
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(791..808)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattcggc ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct
      181 gctctgggtt ccaggttcca ctggtgacta tccatatgat gttccagatt atgctggggc
      241 ccagccggcc agatctgact acaaggatga cgacgataaa tctagacctg gggagaggcc
      301 tttccagtgc cggatttgca tgcggaactt cagcacgggt cagattttgg acaggcacac
      361 caggactcac actggcgaga aaccatttca gtgtaggatc tgtatgagga atttctcagt
      421 tgcccactcc ctgaaaagac acctgcgcac acatactggc gaaaaacctt tccaatgtcg
      481 catatgcatg cgcaacttct cagacccaag caatctccgc aggcatctga agactcacct
      541 gaggggatcc caactagtca aaagtgaact gccgcggctg caggtcgacg aacaaaaact
      601 catctcagaa gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc
      661 acactccttg ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac
      721 catcatctcc cttatcatcc tcatcatgct ttggcagaag aagccacgtt aggcggccgc
      781 tcgagatcag cctcgactgt gccttctagt tgccagccat ctgtt
//
