LOCUS       sequence-60475-e       13085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-XOPS-tdTOM-MOPS-GFP
FEATURES             Location/Qualifiers
     source          1..13085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2474..2707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2969..3086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4543..4557
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      4564..4573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4570..6000
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     intron          6597..6693
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             6758..7471
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     polyA_signal    8438..8662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8795..8814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(9835..9852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10006..10594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10086..10105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10765..11556,11557..11625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11388..11407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11626..11730)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11798..11816
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11867..11889)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11975..12113
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12028..12047
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg ccgcacccgt gtgcttttct ctctttttct ctgacactga
      481 ttttggtgca aaagctgttg ttcgtgtgag cccacagaaa attgtaagtg aagtggatta
      541 ggagcttaca ccgtcacgga gcaaattctg ccctcctaat cttctgctgg gctgtaatct
      601 gcaagacttt cccattttac ctcctttcta ttttaaacct gaatgcttaa tgctcctggt
      661 gggggtccat cctgctcctc attggctgag ggaggatttg ctggcactta aatggcaccc
      721 tctgctccgt gcctctgtgc tttttggttg ttgtgctttt acatttgccc ggagagagag
      781 aggaacctgg gtccatcacc accactgctg cactgggacc agcacgaggc actttaaagg
      841 tgagaaggag aacttcaaag ccccaaagct ttagaactca atcatcaacc tcagaactct
      901 tctatcaacc tcagaactca ccatccgacc ccaggactca cccaccgacc tcaaaactca
      961 tccattgacc tcagaactca tccaccgacc tcagaactca tccaccgacc tccagttttg
     1021 gctgcagagt gactggagcg cgtaactaga gatccctcag acccttttag tcagtgtgga
     1081 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc cagaggagat
     1141 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     1201 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     1261 cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta aggccagggg
     1321 gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1381 cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg ggacagctac
     1441 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca atagcagtcc
     1501 tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta gataagatag
     1561 aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac acaggaaaca
     1621 acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa atggtacatc
     1681 aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca
     1741 gcccagaagt aatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa
     1801 ataccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca
     1861 tcaatgagga agctgcagaa tgggatagat tgcatccagt gcatgcaggg cctattgcac
     1921 caggccagat gagagaacca aggggaagtg acatagcagg aactactagc aaattaagag
     1981 aacaatttgg aaataataaa acaataatct ttaagcaatc ctcaggaggg gacccagaaa
     2041 ttgtaacgca cagttttaat tgtggagggg aatttttcta ctgtaattca acacaactgt
     2101 ttaatagtac ttggtttaat agtacttgga gtactgaagg gtcaaataac actgaaggaa
     2161 gtgacacaat cacactccca tgcagaataa aacaatttat aaacatgtgg caggaagtag
     2221 gaaaagcaat gtatgcccct cccatcagtg gacaaattag atgttcatca aatattactg
     2281 ggctgctatt aacaagagat ggtggtaata acaacaatgg gtccgagatc ttcagacctg
     2341 gaggaggcga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     2401 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     2461 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     2521 gcgcacggtc aatgacgctg acggtacagg ccagacaatt attgtctgat atagtgcagc
     2581 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     2641 ggggcatcaa acagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     2701 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     2761 atgctagttg gagtaataaa tctctggaac agatttggaa taacatgacc tggatggagt
     2821 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2881 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggct tgcggccgga
     2941 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     3001 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     3061 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     3121 gggcggccgc agatctttat acattgctca caaatgagtt gaactggcag ctcacgaatg
     3181 ggacatggag ctgtcattta ctatgctccc caatgcaagt gctagagcac taaggggtgc
     3241 aaaaagagag ctccttcgtg ctaattctaa aagcattgat cctggggtcc agctgtgctg
     3301 tggaagggaa agggtcaata aaggggacat ctgcaatctt ccccttcccc acatgaatac
     3361 agtgctgctt gatgcaggaa ctgatgaatc ccggtctctc tgctccattt ttgagtacag
     3421 agacttgtga aatctagaga actgcaagac atgattcaaa gtgctaagtg caaaaaacaa
     3481 tggtggttta tatctgtctt tgtactttac acttgttcac ttacagtccc tacaattgtg
     3541 tctagtgcag tggtccccaa ccagtagctc gttagtaaca tgttgctccc ccaacccctt
     3601 ggatgttgct cccaatggcc tcaaaacaat tgcttatttt tcaattccag gcaagttttg
     3661 gttgcataaa aaccaggtct atggccaaac agagccccct ctgggctgca aatccacaaa
     3721 gggctaccaa atagacaatc atattcttta ttaggcaccc caagggcttt tttcacgctt
     3781 gtgctgctcc gcaactcttt ttacatttga atgtggctta tgggttaaaa aggtgcaaca
     3841 caaacaaata atctattatt tacacactag tcaagactgg tgctcagctg tggtttgaag
     3901 attctaattc aatgaactaa tggtaaccag ggccggattt ggatttctgc agcccctagg
     3961 ccatgcggtc ctaacgtctg tccacgacga gtcttattgc catccacccg caactcccgc
     4021 aagtgcaaat tttggagcac tggtgctctt cagcaagtgg ctgggcggca tgccgtccct
     4081 aaaagttcgc cgccctaggc acaggccttt gtggcctctc cacaaatcca agcctgatgg
     4141 taactaaatg tagagggaac tgagtaaacc ccaaaaatgg ctgccctggc tcctacaata
     4201 tggaattatc tcctgtaggt cagacctgga tttcttcctg tcacttttaa atacactttc
     4261 ttcttgtgtg tttaacagag agagagattg acaggtgtag acttaatacg tttaagggaa
     4321 gccaattaac actttgcaat tttagcttgg attacagtga ttaatagtgc gctaaatcct
     4381 ttgttgctga cgctgggggt tgcaagctta ctccaggtgg gactttaaaa ggacgagggg
     4441 acagtgggtc atactgtaga acagcttcag ttgggatcac aggcttctag ggatctcgag
     4501 gctagcgtcg atcgacccgg gatcgatagt ttaaacctgt acgacgatga cgataaggat
     4561 cccgccacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
     4621 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     4681 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     4741 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     4801 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4861 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     4921 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     4981 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     5041 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     5101 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     5161 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     5221 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     5281 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     5341 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     5401 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     5461 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     5521 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     5581 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     5641 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     5701 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     5761 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     5821 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     5881 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5941 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa
     6001 gaattcgaag cttgatccgg ctgctaacaa agcccgaaag gagtcgacgg ttcctagatc
     6061 tgaattcggt accgtcaagt gagccattgt cagggcttgg ggactggata agtcaggggg
     6121 tctcctggga agagatggga taggtgagtt caggaggaga cattgtcaac tggagccatt
     6181 gtggagaagt gaatttaggg cccaagggtt ccagtcgcag cctgaggcca ccagactgac
     6241 atggggagga aatcccagag gactctgggg cagacaagat gagacaccct ttcctttctt
     6301 tacctaaggg cctccacccg atgtcacctt ggcccctctg caagccaatt aggccccggt
     6361 ggcagcagtg ggattagcgt tagtatgata tctcgcggat gctgaatcag cctctggctt
     6421 agggagagaa ggtcacttta taagggtctg gggggggtca gtgcctggag ttgcgctgtg
     6481 ggagccgtca gtggctgagc tcgccaagca gccttggtct ctgtctacga agagcccgtg
     6541 gggcagcctc tagaggatcc ggtactcgag gaactgaaaa accagaaagt taactggtaa
     6601 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
     6661 ctgctcctca gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc
     6721 taaaagctgc ggaattgtac ccgcggccgc cgccaccatg agcaagggcg aggaactgtt
     6781 cactggcgtg gtcccaattc tcgtggaact ggatggcgat gtgaatgggc acaaattttc
     6841 tgtcagcgga gagggtgaag gtgatgccac atacggaaag ctcaccctga aattcatctg
     6901 caccactgga aagctccctg tgccatggcc aacactggtc actaccctga cctatggcgt
     6961 gcagtgcttt tccagatacc cagaccatat gaagcagcat gactttttca agagcgccat
     7021 gcccgagggc tatgtgcagg agagaaccat ctttttcaaa gatgacggga actacaagac
     7081 ccgcgctgaa gtcaagttcg aaggtgacac cctggtgaat agaatcgagc tgaagggcat
     7141 tgactttaag gaggatggaa acattctcgg ccacaagctg gaatacaact ataactccca
     7201 caatgtgtac atcatggccg acaagcaaaa gaatggcatc aaggtcaact tcaagatcag
     7261 acacaacatt gaggatggat ccgtgcagct ggccgaccat tatcaacaga acactccaat
     7321 cggcgacggc cctgtgctcc tcccagacaa ccattacctg tccacccagt ctgccctgtc
     7381 taaagatccc aacgaaaaga gagaccacat ggtcctgctg gagtttgtga ccgctgctgg
     7441 gatcacacat ggcatggacg agctgtacaa gtgagcggcc gcggggatcc agacatgata
     7501 agatacattg atgagtttgg acaaaccgtc gacccgggat ccgcggacta gtgaattcca
     7561 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg
     7621 cgagggggat ctcgactcta gaagcttggt acctttaaga ccaatgactt acaaggcagc
     7681 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7741 gtttcgagtt ggcgcgcctg ggagctcacg gggacagccc cccccaaagc ccccagggat
     7801 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
     7861 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
     7921 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
     7981 tgggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac aggcgcgcct
     8041 gggagctcac ggggacagcc ccccccaaag cccccaggga tgtaattacg tccctccccc
     8101 gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc
     8161 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt
     8221 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctgggggata cggggaaaaa
     8281 gctttaggct gaaagagaga tttagaatga caggcgcgcc aactcgaatt cattgcagac
     8341 tttcactgaa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agctcgactg tgccttctag ttgccagcca
     8461 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8521 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8581 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8641 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     8701 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8761 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8821 ctcgccacgt tcgccggctg tgcgggagaa cggagttcta ttatgactca aatcagtctc
     8881 cccaagcatt cggggatcag agtttttaag gataacttag tgtgtagggg gccagtgagt
     8941 tggagatgaa agcgtaggga gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg
     9001 gggccacaag atcggatgag ccagtttatc aatccggggg tgccagctga tccatggagt
     9061 gcagggtctg caaaatatct caagcactga ttgatcttag gttttacaat agtgatgtta
     9121 ccccaggaac aatttgggga aggtcagaat cttgtagcct gtagctgcat gactcctaaa
     9181 ccataatttc ttttttgttt tttttttttt atttttgaga cagggtctca ctctgtcacc
     9241 taggctggag tgcagtggtg caatcacagc tcactgcagc ctcaacgtcg taagctcaag
     9301 cgatcctccc acctcagcct gcctggtagc tgagactaca agcgacgccc cagttaattt
     9361 ttgtattttt ggtagaggca gcgttttgcc gtgtggccct ggctggtctc gaactcctgg
     9421 gctcaagtga tccagcctca gcctcccaaa gtgctgggac aaccggggcc agtcactgca
     9481 cctggcccta aaccataatt tctaatcttt tggctaattt gttagtccta caaaggcagt
     9541 ctagtcccca ggcaaaaagg gggtttgttt cgggaaaggg ctgttactgt ctttgtttca
     9601 aactataaac taagttcctc ctaaacttag ttcggcctac acccaggaat gaacaaggag
     9661 agcttggagg ttagaagcac gatggaattg gttaggtcag atctctttca ctgtctgagt
     9721 tataattttg caatggtggt tcaaagactg cccgcttctg acaccagtcg ctgcattaat
     9781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg cttcctcgct
     9841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10201 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10441 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    11761 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    11821 tcgtcttcaa gaactgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    11881 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    11941 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    12001 atagcggagt gtactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    12061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    12121 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    12181 gccagcgcgg ggaggcagag attgcagtaa gctgagatcg cagcactgca ctccagcctg
    12241 ggcgacagag taagactctg tctcaaaaat aaaataaata aatcaatcag atattccaat
    12301 cttttccttt atttatttat ttattttcta ttttggaaac acagtccttc cttattccag
    12361 aattacacat atattctatt tttctttata tgctccagtt ttttttagac cttcacctga
    12421 aatgtgtgta tacaaaatct aggccagtcc agcagagcct aaaggtaaaa aataaaataa
    12481 taaaaaataa ataaaatcta gctcactcct tcacatcaaa atggagatac agctgttagc
    12541 attaaatacc aaataaccca tcttgtcctc aataatttta agcgcctctc tccaccacat
    12601 ctaactcctg tcaaaggcat gtgccccttc cgggcgctct gctgtgctgc caaccaactg
    12661 gcatgtggac tctgcagggt ccctaactgc caagccccac agtgtgccct gaggctgccc
    12721 cttccttcta gcggctgccc ccactcggct ttgctttccc tagtttcagt tacttgcgtt
    12781 cagccaaggt ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga aagagaccag
    12841 ctttacaggg ggtttatcac agtgcaccct gacagtcgtc agcctcacag ggggtttatc
    12901 acattgcacc ctgacagtcg tcagcctcac agggggttta tcacagtgca cccttacaat
    12961 cattccattt gattcacaat ttttttagtc tctactgtgc ctaacttgta agttaaattt
    13021 gatcagaggt gtgttcccag aggggaaaac agtatataca gggttcagta ctatcgcatt
    13081 tcagg
//
