LOCUS       sequence-60480-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     435..458
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     479..495
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     479..495
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctcgtgaca gccctccgaa
       61 ggaagactct cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg
      121 cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga
      181 agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatgaacgaa tcaaattaac
      241 aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg
      301 aagcgatgat ttttgatcta ttaacggata tataaatgca aaaactgcat aaccacttta
      361 actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc
      421 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccggattc
      481 tagaactagt ggatcccccg ggaaaaaaat ggacaagaag tactccattg ggctcgatat
      541 cggcacaaac agcgtcggtt gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa
      601 attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct
      661 cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag
      721 atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc
      781 taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa
      841 aaa
//
