LOCUS       sequence-60482-e         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Jang CW, Magnuson T
  TITLE     A Novel Selection Marker for Efficient DNA Cloning and 
            Recombineering in E. coli.
  JOURNAL   PLoS One. 2013;8(2):e57075. doi: 10.1371/journal.pone.0057075. Epub 
            2013 Feb 20.
  PUBMED    23437314
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e57075. doi"; pages: " 
            10.1371/journal.pone.0057075. Epub 2013 Feb 20"
COMMENT     Sequence Label: pF2
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..125
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     42..58
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     42..58
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(92..108)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(137..155)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(176..192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(189..211)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    200..216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(224..230,231..248,249..254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    269..290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(407..424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tactataggg cgaattggag ctccaccgcg gtggcggccg ctctagaact agtggatccc
       61 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
      121 gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
      181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgt
//
