LOCUS       sequence-60483-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(44..291)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     257..275
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(307..327)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg
       61 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
      121 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
      181 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
      241 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgagaag
      301 agatcacacc ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag
      361 gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac
      421 ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc
      481 aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat
      541 gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag
      601 gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg
      661 ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata
      721 gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt
      781 ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga
      841 gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag
      901 aaagtcgatg gggttt
//
