LOCUS       sequence-60487-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggccgtttc tgacagagta aaattcttga gggaactttc accattatgg gaaatggttc
       61 aagaaggtat tgacttaaac tccatcaaat ggtcaggtca ttgagtgttt tttatttgtt
      121 gtattttttt ttttttagag aaaatcctcc aatatataaa ttaggaatca tagtttcatg
      181 attttctgtt acacctaact ttttgtgtgg tgccctcctc cttgtcaata ttaatgttaa
      241 agtgcaattc tttttcctta tcacgttgag ccattagtat caatttgctt acctgtattc
      301 ctttacatcc tcctttttct ccttcttgat aaatgtatgt agattgcgta tatagtttcg
      361 tctaccctat gaacatattc cattttgtaa tttcgtgtcg tttctattat gaatttcatt
      421 tataaagttt atgtacaaat atcataaaaa aagagaatct ttttaagcaa ggattttctt
      481 aacttcttcg gcgacagcat caccgacttc ggtggtactg ttggaaccac ctaaatcacc
      541 agttctgata cctgcatcca aaaccttttt aactgcatct tcaatggcct taccttcttc
      601 aggcaagttc aatgacaatt tcaacatcat tgcagcagac aagatagtgg cgatagggtt
      661 gaccttattc tttggcaaat ctggagcaga accgtggcat ggttcgtaca aaccaaatgc
      721 ggtgttcttg tctggcaaag aggccaagga cgcagatggc aacaaaccca aggaacctgg
      781 gataacggag gcttcatcgg agatgatatc accaaacatg ttgctggtga ttataatacc
      841 attta
//
