LOCUS       sequence-60490-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Lipovsek D, Antipov E, Armstrong KA, Olsen MJ, Klibanov AM, Tidor B,
            Wittrup KD
  TITLE     Selection of horseradish peroxidase variants with enhanced 
            enantioselectivity by yeast surface display.
  JOURNAL   Chem Biol. 2007 Oct;14(10):1176-85.
  PUBMED    17961829
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2007-10"; volume: "14(10)"; pages: "1176-85"
COMMENT     Sequence Label: pCT2-HRP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             317..328
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             329..355
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccggatcgaa ttcctacttc atacattttc aattaagatg cagttacttc gctgtttttc
       61 aatattttct gttattgctt cagttttagc acaggaactg acaactatat gcgagcaaat
      121 cccctcacca actttagaat cgacgccgta ctctttgtca acgactacta ttttggccaa
      181 cgggaaggca atgcaaggag tttttgaata ttacaaatca gtaacgtttg tcagtaattg
      241 cggttctcac ccctcaacaa ctagcaaagg cagccccata aacacacagt atgtttttaa
      301 ggacaatagc tcgacgattg aaggtagata cccatacgac gttccagact acgctctgca
      361 ggctagtggt ggaggaggct ctggtggagg cggtagcgga ggcggagggt cggctagcca
      421 acttacccct accttctacg acaattcatg tcctaacgtc tcaaacatag tacgggacac
      481 tattgtcaat gagttacgat cggaccctag gatcgccgcg agcatccttc gtcttcactt
      541 ccacgactgc tttgttaacg gttgtgacgc atcgatcttg ttagacaaca caacatcatt
      601 tcgaacagag aaagatgcgt ttgggaacgc aaactcggcg cgcggatttc ctgtgattga
      661 cagaatgaag gcggccgtgg agagtgcatg cccaagaact gtatcctgcg cagatctgct
      721 taccattgca gctcaacaat ctgttacttt ggcaggaggt ccctcttgga gggttccttt
      781 gggacgtcga gacagcctac aagcattttt agatctcgcg aatgcgaatc ttccagctcc
      841 attcttcaca cttccacaac ttaaggattc ttttagaaat gt
//
