LOCUS       sequence-60493-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Jang CW, Magnuson T
  TITLE     A Novel Selection Marker for Efficient DNA Cloning and 
            Recombineering in E. coli.
  JOURNAL   PLoS One. 2013;8(2):e57075. doi: 10.1371/journal.pone.0057075. Epub 
            2013 Feb 20.
  PUBMED    23437314
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e57075. doi"; pages: " 
            10.1371/journal.pone.0057075. Epub 2013 Feb 20"
COMMENT     Sequence Label: pF2-DTA
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     200..219
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ttggtaggat ccactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
       61 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
      121 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
      181 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
      241 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
      301 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
      361 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
      421 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
      481 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
      541 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
      601 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
      661 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
      721 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
      781 ttaattttga aacccg
//
