LOCUS       sequence-60498-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Zhou W, Dosey TL, Biechele T, Moon RT, Horwitz MS, Ruohola-Baker H
  TITLE     Assessment of hypoxia inducible factor levels in cancer cell lines 
            upon hypoxic induction using a novel reporter construct.
  JOURNAL   PLoS One. 2011;6(11):e27460. doi: 10.1371/journal.pone.0027460. Epub
            2011 Nov 23.
  PUBMED    22132102
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27460. doi"; pages: " 
            10.1371/journal.pone.0027460. Epub 2011 Nov 23"
COMMENT     Sequence Label: HBR-6U
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(95..126)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    317..434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(696..740)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atcaagggtc cccaaactca ccctgaagtt ctcaggatcg atccggagct ttttgcaaaa
       61 gcctagtttt taccaacagt accggaatgc caagctggaa ttcgagcttc cattatatac
      121 cctctagagt ctagatctcg agcccggcgt cgtcggtccg attgctacga agcttcgtgc
      181 tgtgtgagac agcacgtaca cgtagcccac gtacacgtcg tacacgtaca cgagtagtct
      241 agagtgctgt gtgagacagc acgtacacga tcgccacgta cacgtgaagc acgtacacgg
      301 cgtgttctga gtcgataaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
      361 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
      421 cttttctttt aaaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
      481 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
      541 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
      601 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
      661 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
      721 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
      781 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaa
//
