LOCUS       sequence-60500-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  TITLE     Callaway lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-HTB
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(74..107)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc tattagcaat
       61 atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc gcgccctatt
      121 acttgtacat cttacagtct ggtctcaccc ccactcttgt gtgattccca tgaagatatg
      181 atcttcccgc tttggggagt gactgacacc tccctccctg tccctctgag attgtgttgc
      241 gtaggttctg atcgattgac tcttctacaa catgtcatca ggaaaattat caacatcaag
      301 gcagtcaggg cccctgcact cagtaataca tacttccccc agttcgggag acccaagtca
      361 actcctgaga cctgattgtg cacatcggga aggtgaactt caacaaaatc ctcagcctcg
      421 tcaccgtcct tgaaaacggt agacgggtct gccagggggt gcacaagggg gataaccgag
      481 gattccaaca actccatatg ttgctggagg agggatgatt gcatctctgg gattaagaca
      541 ttgccgtcag gtcctaatat tataccattg aaaaacaccc cgttcacatg aggatgacac
      601 ctccccccaa ctcttaaaca cccttttgaa gggaggatct cattccaagt tctgactgac
      661 ttgtagtgag catcggcttc catcaaggtc ttgtttg
//
