LOCUS       sequence-60502-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     Callaway lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-HTB
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(36..413)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     protein_bind    429..462
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    513..546
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(624..644)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctcgccttgc tcaccatggt ggcgaccggt ggatccttag cgctggtgta cttggtgatg
       61 gccttagtac cctcggacac ggcgtgcttg gccaactccc caggcagcag caggcgcacg
      121 gccgtctgga tctccctgga ggtgatggtc gagcgcttgt tgtaatgcgc caggcgggaa
      181 gcctcacctg cgatgcgctc gaaaatgtcg ttcacaaacg aattcatgat gcccatggcc
      241 ttggacgaaa tgccggtgtc agggtggacc tgcttcagaa ccttgtacac atagatggaa
      301 tagctctcct tgcggctgcg cttgcgcttc ttgccgcctt tcttctgcgc cttagtcacc
      361 gccttcttgg agcccttttt cggggcggga gcagacttcg ctggctctgg catggtggcg
      421 gctctagcat aacttcgtat aaagtatcct atacgaagtt atttgcctta acccagaaat
      481 tatcactgtt attctttaga atggtgcaaa gaataacttc gtataatgta tgctatacga
      541 agttatgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
      601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
      661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
      721 tcctggttgc tgtctcttta tgaggagttg tgggcccgtt gtcaggcaa
//
