LOCUS       sequence-60505-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     Callaway lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-HTB
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..83
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             573..632
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 acgagctgta caagaagctt ggtagtggag agggcagagg aagtctgcta acatgcggtg
       61 acgtcgagga gaatcctggc ccaatcgatg cgcggctgct gcccgcgctg ctgctgctgc
      121 tgctgcccgg taacgtgacc ggtaacgggt ccggtaacgg ttctttgtcc cgttgccccc
      181 ccggtcagtt ccgctgctcg gagccgcccg gtgcccacgg ggagtgttac ccgcaggact
      241 ggctgtgcga cggacacccc gactgcgacg acgggcggga cgagtggggc tgcgggacca
      301 gcgcgatccc cgcggtgccc acggacaacg gcacagaggc tcccactgcc cctgctcctg
      361 gacgtgctct gccagccagg aatcacggcc gcatgtggat gctgatcact gcagtgctcc
      421 tgtgctgcct ggtagctgtg ggtggtatcg ctgcatgggg gaagtccaaa gcaaaaagca
      481 ggtctgacat cttcagtctt gaaagcgcat ccaaggagct gctggtgcct gacaagagcc
      541 aggcagactt gttctccgct agcggtagtg gtcaatgtac taactacgct ttgttgaaac
      601 tcgctggcga tgttgaaagt aaccccggtc cttccggaat ggttcctcag gctctcctgt
      661 ttgtacccct tctggttttt ccattgtgtt ttgggaaatt ccctatttac acgataccag
      721 acaagcttgg tccctggagt ccgattgaca tacatcacct cagctgccca aacaatttgg
      781 tagtggagga cgaaaggatg caccaacctg tcagggttct cctacatgga acttaaaagt
      841 tggatacatc ttagccataa aagtga
//
