LOCUS       sequence-60513-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Erard M, Fredj A, Pasquier H, Beltolngar DB, Bousmah Y, Derrien V, 
            Vincent P, Merola F
  TITLE     Minimum set of mutations needed to optimize cyan fluorescent 
            proteins for live cell imaging.
  JOURNAL   Mol Biosyst. 2013 Feb 2;9(2):258-67. doi: 10.1039/c2mb25303h. Epub 
            2012 Nov 28.
  PUBMED    23192565
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c2mb25303h"; journalName: "Mol Biosyst"; date: "2013-02-2- 
            2"; volume: "9"; issue: "2"; pages: "258-67"
COMMENT     Sequence Label: pAquaN1
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..105
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(113..115,116..118,119..832)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(158..179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(419..438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     766..787
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
      121 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      181 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      241 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      301 caccctgagc tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      361 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      421 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      481 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      541 gtacaactac atcagcggca acgtctatat caccgccgac aagcagaaga acggcatcaa
      601 ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      661 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      721 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      781 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
      841 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      901 ctcccacacc tccccctgaa cctg
//
