LOCUS       sequence-60515-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH094
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..62)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(78..101)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(117..140)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ggtacctatt aagcccatga gtccatatgc tgtctctcga attttgcagc cgctgtctct
       61 ttactaccac ctcctccagg tccttggaag agaacttcca atgatcctcc acctcctttc
      121 tcaaattgtg gatgtgacca tcgcgaacct cctcctcccg atccgcctcc tccactacca
      181 ccacctccgg atacgtagag ctcgtccata ccatcagttg agtttctagc tactgctgac
      241 tcatacacag tcactttatt gtaatcttta tcatggttca aaatttcaat tcggtgatcc
      301 acaaagtgat agtctggcaa tttcacagct ttcttagctc tgtagattgt cttaaagtcc
      361 actcgatgat gtccacctcc ttccaacaac agtttatgct tcacgtcacc tttcaaaact
      421 ccatctctga catcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
      481 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
      541 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
      601 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
      661 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
      721 ccgatgccgc cggaagcg
//
