LOCUS       sequence-60522-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH066
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(160..264)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             407..451
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             467..490
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             506..529
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
       61 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
      121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
      301 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      361 cagcccctca gcgcggccgc tagcggatcc ttaaaaaacc atggttaaag aaacagcagc
      421 agcgaaattt gaaagacaac atatggatag tggaggcggt ggaagtttgg aagttctttt
      481 ccaaggaccg ggcggaggag gctcttggtc gcatccacag ttcgaaaaag ccatggccgg
      541 cggtggtgga tcgggaggag gaggttcggg tggcggagga tcgggcgcct caaaaggaga
      601 agaaacgaca atgggtgtta tcaagccaga catgaagatc aagcttaaaa tggagggtaa
      661 tgttaatggt cacgccttcg tcattgaggg aga
//
