LOCUS       sequence-60534-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LOV (N terminal domain of FKF1) fused to VP16, part of a 
            light-inducible transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Yazawa M, Sadaghiani AM, Hsueh B, Dolmetsch RE
  TITLE     Induction of protein-protein interactions in live cells using light.
  JOURNAL   Nat Biotechnol. 2009 Oct;27(10):941-5. doi: 10.1038/nbt.1569. Epub 
            2009 Oct 4.
  PUBMED    19801976
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1569"; 
            journalName: "Nat Biotechnol"; date: "2009-10"; volume: "27"; issue:
            "10"; pages: "941-5"
COMMENT     Sequence Label: pCDNA3-LOV-VP16
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             668..778
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(822..840)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(823..840)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(860..877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccgccacca tggcttaccc
      121 atacgatgtt ccagattacg ctgcgagaga acatgcgatc ggagaagcca ccggaaaacg
      181 aaagaaacgc ggcagagttg aagaagcaga agaatactgt aacgatggaa tcgaagaaca
      241 agtagaggat gagaagcttc cgttagaggt tgggatgttc tattacccaa tgactccgcc
      301 ttcgttcatt gtttccgatg ctctggagcc agattttcct ttgatctatg tcaacagagt
      361 cttcgaagtc ttcactggct atcgtgccga tgaagttctt ggtcgtaact gtcgattcct
      421 acagtacaga gatcctcgag ctcaaaggcg tcacccattg gttgatcctg tggttgtatc
      481 tgagattagg agatgtcttg aagaaggtat tgaattccaa gacgagcttc ttaatttcag
      541 aaaagatggt actcctttgg ttaacagact acggcttgct ccaatacgtg acgatgatgg
      601 aaccattaca cacgtaattg ggatacaggt cttctctgaa acgggtaccg aaagcggcgg
      661 gccggccgac gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga
      721 ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc
      781 cgggtaagcg gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
      841 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      901 ccctcccccg tgccttcct
//
