LOCUS       sequence-60536-e         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH051
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(45..65)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctatttgta tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa
       61 ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt
      121 aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt
      181 ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagttc tgaaaattta
      241 atcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
      301 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
      361 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
      421 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
      481 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcggg
//
