LOCUS       sequence-60537-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH051
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(173..277)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(440..468)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      301 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      361 ccgctacact tgccagcccc tcagcgcggc cgctagcgga tccttaaaaa tgagtaaagg
      421 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      481 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      541 taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat
      601 actaactaac cctgattatt taaattttca gccaacactt gtcactactg ctgtcgagat
      661 atgacggtgt tcacaaagtt ccttaaattt tacttttggt tacatatttt
//
