LOCUS       sequence-60550-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: pTAL_GFP
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..80
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             87..107
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggccgccac catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
       61 acaaggatga cgatgacaag atggccccca agaagaagag gaaggtgggc attcacgggg
      121 tgccgtctag agtagatttg agaactttgg gatattcaca gcagcagcag gaaaagatca
      181 agcccaaagt gaggtcgaca gtcgcgcagc atcacgaagc gctggtgggt catgggttta
      241 cacatgccca catcgtagcc ttgtcgcagc accctgcagc ccttggcacg gtcgccgtca
      301 agtaccagga catgattgcg gcgttgccgg aagccacaca tgaggcgatc gtcggtgtgg
      361 ggaaacagtg gagcggagcc cgagcgcttg aggccctgtt gacggtcgcg ggagagctga
      421 gagggcctcc ccttcagctg gacacgggcc agttgctgaa gatcgcgaag cggggaggag
      481 tcacggcggt cgaggcggtg cacgcgtggc gcaatgcgct cacgggagca cccctcaacc
      541 tgaccgagac gttagtctag tcgtctcagg agtcaatcgt ggcccagctt tcgaggccgg
      601 accccgcgct ggccgcactc actaatgatc atcttgtagc gctggcctgc ctcggcggac
      661 gacccgcctt ggatgcggtg aagaaggggc tcccgcacgc gcctgcattg attaagcgga
      721 ccaacagaag gattcccgag aggacatcac atcgagtggc aggatcccaa ctagtcaaaa
      781 gtgaactgga ggagaagaaa tctgaacttc gtcataaatt gaat
//
