LOCUS       sequence-60551-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: pTAL_GFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..73)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(89..676)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNPTQDRILEMKV
                     MEFFMKVYGYRGEHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tctcgccctt gctcaccatt gggccgggat tctcctccac gtcaccgcat gttagaagac
       61 ttcctctgcc ctctccgccg ccggacctaa agtttatctc gccgttatta aatttccgtc
      121 ttacttcctc taaggttaat gtgccggctt taatcatttc tccaccaatt aaaagctctt
      181 ctacactaag aacagctcca ttacaattag tgatatgatt taatcgtgta agctgagctt
      241 tgtagtttcc tttaaagtga ccactcacaa ataaaaactt aaattccgtt acagaagatg
      301 gatagacttt ccaccattca ttagggttga gatgtttgtc tcgtgtttga ttttcttcga
      361 catatcgctc catttcatct gcttggccaa ttggcagatt ataacctccg ctataagctt
      421 tagtatccac gatcacaccg taatcaatag gagatccgac agtataaatt gctccgtccg
      481 gtttccttga tccacccaaa tgttcacctc tatatccata aactttcata aaaaattcca
      541 ttaccttcat ttcaaggatt ctatcctgag tgggatttct ggcaatttca attaattcaa
      601 tatattcatg aggcacatat ttcaatttat gacgaagttc agatttcttc tcctccagtt
      661 cacttttgac tagttgggat cctgccactc gatgtgatgt cctctcggga atccttctgt
      721 tggtccgctt aatcaatgca ggcgcgtgcg ggagcccctt cttcaccgca tccaaggcgg
      781 gtcgtccgcc gaggcaggcc agcgctacaa gatgatcatt agtgagtgcg gccagcgcg
//
