LOCUS       sequence-60552-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: pTAL_RFP
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    34..155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(45..64)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     99..118
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(482..501)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     751..769
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag tttggacaaa
       61 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      121 tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg
      181 agatccccgc gctgcagtca cacgtgcttg tacagctcgt ccatgccgcc ggtggagtgg
      241 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      301 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
      361 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
      421 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
      481 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
      541 tagatgaact cgccgtcctg gagggaggag tcctgggtca cggtcaccac gccgccgtcc
      601 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
      661 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
      721 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
      781 ccctc
//
