LOCUS       sequence-60553-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: pTAL_RFP
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..165)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
       61 catggactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca ttgggccggg
      121 attctcctcc acgtcaccgc atgttagaag acttcctctg ccctctccgc cgccggacct
      181 gaaattgatt tcaccattgt tgaacttgcg gcgcacctct tccagggtca gggtaccagc
      241 cttgatcatc tcgccgccga tcagcagttc ctccacggac agcacggcgc cgttgcagtt
      301 ggtcttgcgg ttcaggcggg tcagttgggc cttataattg cccttgaagt ggccggacac
      361 gaacaggaat ttgaactcgg tcacggagga ggggtacacc ttccaccact cgttgggatt
      421 aatgtgctta ttgcgggtct ggttctcctt cacgtagcgt tgcatctcgt cggcctgacc
      481 gatgggtaag ttgtagccgc cggagtaggc cttggtgtcg acaatgacgc catagtcgat
      541 gggggagccc acggtgtaga tggcgccatc gggcttgcgg gagccgccca ggtgctcgcc
      601 gcggtagccg tacaccttca tgaagaactc catcactttc atctccagga tgcggtcttg
      661 ggtggggttg cgggcgatct cgatcagctc gatgtactcg tggggcacgt acttgagctt
      721 gtggcgcagc tcgctctttt tttcttcgag ctcggacttc accagctggg atcctgccac
      781 tcgatgtgat gtcctctcgg gaatccttct gttggtccgc ttaatcaatg caggcgcgtg
      841 cgggagcccc ttcttcaccg catccaaggc gggtcgtccg ccgaggcagg ccagcgctac
      901 aagatgatca ttagtgagtg cggccagcgc gggg
//
