LOCUS       sequence-60561-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-TCGA
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(606..624)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(607..624)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(642..658)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(642..658)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(655..677)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    666..682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(690..696,697..714,715..720))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    735..756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(873..890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttgtc ctagcgcgtc tcgcttaacc ccagagcagg tcgtggccat
      121 tgcctcgaat ggagggggca aacaggcgtt ggaaaccgta caacgattgc tgccggtgct
      181 gtgccaagcg cacgggctca ccccagagca ggtcgtggcg atcgcaagcc acgacggagg
      241 aaagcaagcc ttggaaacag tacagaggct gttgcctgtg ctgtgccaag cgcacggcct
      301 caccccagag caggtcgtgg caatcgcgag caataacggc ggaaaacagg ctttggaaac
      361 ggtgcagagg ctccttccag tgctgtgcca agcgcacgga ttaaccccag agcaggtcgt
      421 ggcaatcgcc tccaacattg gcgggaaaca ggcactcgag actgtccagc gcctgcttcc
      481 cgtgctgtgc caagcgcacg ggctcagaga cgtgggcaca ggaaagaagg gcgaattcca
      541 gcacactggc ggccgttact agtggatccg agctcggtac caagcttgat gcatagcttg
      601 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
      661 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      721 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      781 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      841 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      901 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      961 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1021 aaaa
//
