LOCUS       sequence-60589-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-AGAA
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(598..614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(598..614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(611..633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    622..638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(646..652,653..670,671..676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    691..712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(829..846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa ttcgccctta
       61 gtatctttcc tgtgcccacg tctctgagcc cgtgcgcttg gcacagcacg ggaagcaggc
      121 gctggacagt ctcgagtgcc tgtttcccgc caatgttgga ggcgattgcc acgacctgct
      181 ctggggttaa tccgtgcgct tggcacagca cgggaagcag gcgctggaca gtctcgagtg
      241 cctgtttccc gccaatgttg gaggcgattg ccacgacctg ctctggggtg aggccgtgcg
      301 cttggcacag cactggaagg agcctctgca ccgtttccaa agcctgtttt ccgccgttat
      361 tgctcgcgat tgccacgacc tgctctgggg tgagtccgtg cgcttggcac agcacgggaa
      421 gcaggcgctg gacagtctcg agtgcctgtt tcccgccaat gttggaggcg attgccacga
      481 cctgctctgg ggttaagcga gacgcgctag gacggcatct atataagggc gaattccagc
      541 acactggcgg ccgttactag tggatccgag ctcggtacca agcttggcgt aatcatggtc
      601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      661 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatc
//
