LOCUS       sequence-60591-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-AGAT
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(602..618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(602..618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(615..637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    626..642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(650..656,657..674,675..680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    695..716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(833..850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattcgcc
       61 cttagtatct ttcctgtgcc cacgtctctg agcccgtgcg cttggcacag caccggcagc
      121 aatcgttgta cggtttccaa cgcctgtttg ccccctccat tcgaggcaat ggccacgacc
      181 tgctctgggg ttaatccgtg cgcttggcac agcacgggaa gcaggcgctg gacagtctcg
      241 agtgcctgtt tcccgccaat gttggaggcg attgccacga cctgctctgg ggtgaggccg
      301 tgcgcttggc acagcactgg aaggagcctc tgcaccgttt ccaaagcctg ttttccgccg
      361 ttattgctcg cgattgccac gacctgctct ggggtgagtc cgtgcgcttg gcacagcacg
      421 ggaagcaggc gctggacagt ctcgagtgcc tgtttcccgc caatgttgga ggcgattgcc
      481 acgacctgct ctggggttaa gcgagacgcg ctaggacggc atctatataa gggcgaattc
      541 cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg gcgtaatcat
      601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atca
//
