LOCUS       sequence-60597-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-AGGA
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(593..609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(593..609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(606..628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    617..633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(641..647,648..665,666..671))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    686..707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(824..841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat tcgcccttga
       61 tgccgtccta gcgcgtctcg cttaacccca gagcaggtcg tggcaatcgc ctccaacatt
      121 ggcgggaaac aggcactcga gactgtccag cgcctgcttc ccgtgctgtg ccaagcgcac
      181 ggactcaccc cagagcaggt cgtggcaatc gcgagcaata acggcggaaa acaggctttg
      241 gaaacggtgc agaggctcct tccagtgctg tgccaagcgc acggcctcac cccagagcag
      301 gtcgtggcaa tcgcgagcaa taacggcgga aaacaggctt tggaaacggt gcagaggctc
      361 cttccagtgc tgtgccaagc gcacggatta accccagagc aggtcgtggc aatcgcctcc
      421 aacattggcg ggaaacaggc actcgagact gtccagcgcc tgcttcccgt gctgtgccaa
      481 gcgcacgggc tcagagacgt gggcacagga aagatactta agggcgaatt ccagcacact
      541 ggcggccgtt actagtggat ccgagctcgg taccaagctt ggcgtaatca tggtcatagc
      601 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      661 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      721 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      781 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      841 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
//
