LOCUS       sequence-60613-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-ATCG
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(599..615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(599..615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(612..634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    623..639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(647..653,654..671,672..677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    692..713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(830..847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga attcgccctt
       61 agtatctttc ctgtgcccac gtctctgagc ccgtgcgctt ggcacagcac tggaaggagc
      121 ctctgcaccg tttccaaagc ctgttttccg ccgttattgc tcgcgattgc cacgacctgc
      181 tctggggtta atccgtgcgc ttggcacagc acaggcaaca gcctctgtac tgtttccaag
      241 gcttgctttc ctccgtcgtg gcttgcgatc gccacgacct gctctggggt gaggccgtgc
      301 gcttggcaca gcaccggcag caatcgttgt acggtttcca acgcctgttt gccccctcca
      361 ttcgaggcaa tggccacgac ctgctctggg gtgagtccgt gcgcttggca cagcacggga
      421 agcaggcgct ggacagtctc gagtgcctgt ttcccgccaa tgttggaggc gattgccacg
      481 acctgctctg gggttaagcg agacgcgcta ggacggcatc tatataaggg cgaattccag
      541 cacactggcg gccgttacta gtggatccga gctcggtacc aagcttggcg taatcatggt
      601 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      661 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      721 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      781 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      841 actcgctgcg
//
