LOCUS       sequence-60614-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-ATCT
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(588..606)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(589..606)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(624..640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(624..640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(637..659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    648..664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(672..678,679..696,697..702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    717..738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(855..872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctgatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc gcccttgccg
       61 tcctagcgcg tctcgcttaa ccccagagca ggtcgtggca atcgcctcca acattggcgg
      121 gaaacaggca ctcgagactg tccagcgcct gcttcccgtg ctgtgccaag cgcacggact
      181 caccccagag caggtcgtgg ccattgcctc gaatggaggg ggcaaacagg cgttggaaac
      241 cgtacaacga ttgctgccgg tgctgtgcca agcgcacggc ctcaccccag agcaggtcgt
      301 ggcgatcgca agccacgacg gaggaaagca agccttggaa acagtacaga ggctgttgcc
      361 tgtgctgtgc caagcgcacg gattaacccc agagcaggtc gtggccattg cctcgaatgg
      421 agggggcaaa caggcgttgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca
      481 cgggctcaga gacgtgggca caggaaagaa gggcgaattc cagcacactg gcggccgtta
      541 ctagtggatc cgagctcggt accaagcttg atgcatagct tgagtattct atagtgtcac
      601 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      661 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      721 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      781 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      841 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
//
