LOCUS       sequence-60615-e       12956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Mao CA, Cho JH, Wang J, Gao Z, Pan P, Tsai WW, Frishman LJ, Klein WH
  TITLE     Reprogramming amacrine and photoreceptor progenitors into retinal 
            ganglion cells by replacing Neurod1 with Atoh7.
  JOURNAL   Development. 2013 Feb;140(3):541-51. doi: 10.1242/dev.085886.
  PUBMED    23293286
REFERENCE   2  (bases 1 to 12956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-02"; volume: "140"; issue: 
            "3"; pages: "541-51"
COMMENT     Sequence Label: Neurod1-Atoh7HA3
FEATURES             Location/Qualifiers
     source          1..12956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2642..2675)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2681..2905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2911..3714)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3022..3041)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3632..3651
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     regulatory      3711..3720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3732..4231)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3780..3799)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4173..4196
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    4248..4281
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(4282..4315)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5139..5219
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             9949..10521
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          10598..10663
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(10768..10788)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10768..10786)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10807..10823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10807..10823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10820..10842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10831..10847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10855..10861,10862..10879,10880..10885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10900..10921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11038..11055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11209..11797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11289..11308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11968..12759,12760..12828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12591..12610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12829..12933)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctagg
      661 cggccgcgga gtctctaact ggcgacagat gggccacttt cttctggcca caaaggggcc
      721 ggaatggagc gctccgcggc atacaaatag gcaggtcacg tggttcccgg ctcttggctg
      781 gaccgggaag accatatggc gcatgccggg gaggaaggag caggggcgga ggtactgtgg
      841 gggtgagggg agtggtggtg gaagggggcg ggaggaaagt tctggggagg ggtgaatgag
      901 ggcgggagtc gttcagtctg gacgcgtgcg cgattgcggg gcgcagggac ctgctcgttg
      961 ctcagctcac ggccccgccc gcgctcagca tcagcaactc ggctatataa ccctagcgcc
     1021 cgcgccaccg ggacacgagg aattcgccca cgcagaaggc aaggtgtccc gaggctccag
     1081 ggttatgaga tcgtcactat tcagaacctt ttaacaacag gtaattatga ttcctatttt
     1141 ttactctccc gagaataaaa gcaataattg aagagctgta ccttggtact ttgcatgcgc
     1201 acatttgtgg agtcggttgt ccctatattt gagatcagtg tccctcctcg taattgccca
     1261 caagtactcg cacgtaatga gtcccagcac tcaagtaccc cctgcaggct ttctaattac
     1321 atttaatttt taatagtata gaagctttta gaatgtttgg ggaggggagt aactgtaatt
     1381 atttgtaagc ggttatttga aagtccccaa agcaaacaac cacttccagt tctcacttat
     1441 tcaggggaga aaaggagaga aagagaaaaa caaaacaaaa caaaacaaac aaacaaaaac
     1501 acctgctgcc catagtttta tttggtgtac agatatttag gctcatcgtg catatgaaac
     1561 tatctatttc aggagaatgt agtactggaa aaccgattgg actctgaaat agcttagctg
     1621 aaatgtaggg agagggggag aaaaggaagg aaattttcct gctacctgtt actgtcccag
     1681 aggcggccaa gtccccatgc tggggccaga cattcagttg ttttactaaa ggctttttac
     1741 agttcttatg agaagcagca ccttcaccat tgccagtcgc tgaccattgt aattactcca
     1801 tcttttaaag ggaggtttga ggcgaacttt gaaaagcccc aataaagagc gggcggccct
     1861 ttcaatgggc tgtttattag agaggagcct tttgcacctg atccccagcg cgtgggagag
     1921 cctcgcctgg gagccagagc ttttgtacaa ggtgggaatt tagcgggggc gtgggggggg
     1981 ggtggtgagg caaagaaaga aaggcaagag agggatggaa gacttgtgcc cacttctgtg
     2041 gccaaatgtg ttgtcgatag gaaaataatt tgatggaaac tacctttcct cctctgtgca
     2101 ttggctctcc cttccatata atcttcttta aaaattgggg tcagtgctag gaaattacgt
     2161 aatggctttt caatttattt aaacctctaa gtagtgcgaa gccagagcag agatgagttc
     2221 agagtattcc atttccaaac tcatcgtttt tgtgagttgg gagtgacttg ggacacagtc
     2281 agaaaaccat gcactctgta cgcatttcca cacagggcac ctggccagat aattgccctg
     2341 cacacagttg agctgatcac taggaaaggc acccatagcc actcatgaca ggcctagtgg
     2401 tacctactac gcagcaccga ggtacagaca cgccagcatg aagcactgcg tttaactttt
     2461 cctggaggca tccattttgc agtggactcc tgtgtatttc tatttgtgtg catttctgta
     2521 ggattaggga gagggagctg aaggcttatc cagcttttaa atatagcggg tggatttccc
     2581 cccctttctt cttctgcttg cctctctccc tgttcaatac aggaagtgga aacttaatta
     2641 aataacttcg tatagcatac attatacgaa gttgtgatat ccatagagcc caccgcatcc
     2701 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     2761 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     2821 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     2881 gatggctggc aactagaagg cacagtcgac tcagaagaac tcgtcaagaa ggcgatagaa
     2941 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3001 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3061 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3121 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
     3181 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     3241 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     3301 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     3361 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3421 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3481 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     3541 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     3601 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     3661 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atggccgatc ccatggtggc
     3721 ggcctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc
     3781 ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg
     3841 agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg
     3901 aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg
     3961 gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac
     4021 gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg
     4081 aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga
     4141 ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca
     4201 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt cggatccgaa gttcctattc
     4261 tctagaaagt ataggaactt cataacttcg tatagcatac attatacgaa gttgtcgtga
     4321 ctgagaaaac cctggcgacc ggtgcgtatt tatcgaattc ggcacgagga tttcgtcacc
     4381 ccgaaaagca gtctcaggct ttcccagaga actggaaagg ctttctatcc ccgaccccca
     4441 agaacgatcc gcagcaaact gcaagaaagt ttcctcctcg ttcaacttta cgtgtttggg
     4501 gtggagtggg gaggaatgac tttttcctga gcatcacttc cactgcatct gggcaaggga
     4561 agggacttcc cagcaggacc caagctaggg tcccacacag tgtcctctgg ccactgactg
     4621 actgcacgtg agtcgctccg tctgtgtctt attcactctt gggagtcctg cagagcggca
     4681 gcgtggatga agtcggcctg caaaccccac ggccctccgg cgggagctcg cggcgcgccc
     4741 ccgtgcgcgg gcgcagccga gcgcgcggtc tcgtgcgcgg ggcccgggcg gctggagagc
     4801 gcggcgcgca ggcgtctggc ggccaacgcg cgcgagcggc gccgcatgca ggggctgaac
     4861 acggcgttcg accggctgcg cagggtggtg ccgcagtggg gccaggacaa gaagctgtcc
     4921 aagtacgaga cactgcagat ggcgctcagc tacatcatcg cgctcacccg catcctagcc
     4981 gaagccgagc gggactgggt cgggctgcgc tgcgagcagc ggggccgcga tcacccctac
     5041 ctccctttcc cgggtgctag gctccaggta gaccctgagc cctatgggca gaggctcttc
     5101 ggcttccagc cggagccctt ccccatggcc agcggtacta cccttacgac gttccagatt
     5161 atgcatatcc atatgatgtg cctgactatg cttaccccta cgacgtcccc gattacgccg
     5221 tcgagtaatt agatccactg tgcgtacagt gactgtcctg tttacagaag gcagcccttt
     5281 tgctaagatt gctgcaaagt gcaaatactc aaagcttcaa gtgatatatg tatttattgt
     5341 cgttactgcc tttggaagaa acaggggatc aaagttcctg ttcaccttat gtattgtttt
     5401 ctatagctct tctattttaa aaataataat acagtaaagt aaaaaagaaa atgtgtacca
     5461 cgaatttcgt gtagctgtat tcagatcgta ttaattatct gatcgggata aaaaaaatca
     5521 caagcaataa ttaggatcta tgcaattttt aaactagtaa tgggccaatt aaaatatata
     5581 taaatatata tttttcaacc agcattttac tacctgtgac ctttcccatg ctgaattatt
     5641 ttgttgtgat tttgtacaga atttttaatg actttttata acgtggattt cctattttaa
     5701 aaccatgcag cttcatcaat ttttatacat atcagaaaag tagaattata tctaatttat
     5761 acaaaataat ttaactaatt taaaccagca gaaaagtgct tagaaagtta ttgcgttgcc
     5821 ttagcacttc tttcttctct aattgtaaaa aagaaaagaa aagaaaaaaa accaacaaat
     5881 tgcacaattt gagcaattca tctcacttta aagtttttcc tgctcgctcc ctaaaataga
     5941 aaccagaccc ataacactca agaggatgaa aaccgaaatg cattccttat caaaacacat
     6001 caattcatta cttgcacaag cttgtaaata catattataa ataaatgcca acacacactc
     6061 ctttaaatca aaagctgctt gactatcaca tacaatttgc actctttctt tttagtcttt
     6121 tacttctttg aattccatga ttttacggag tgtttgaaga tattgatgtt tccagaaaat
     6181 ataaatgcat gattttatac atagtcaaac aaatggtggt ttgtcatcta ttcatgtaat
     6241 aaatctgagc ctaaatttat tcaggttgtt aatgttgggt ttttatacct gtgtagtcag
     6301 ttagtacagt agtttaaata aaattcaaac catcgaattc ataattagaa caatagctgt
     6361 tgcatgtaaa atgcagtcca gaataagtgc tgtttgagat gtgatgctgg tactactgga
     6421 attgacatgt actgtaatct tgtttgtaat cctgtgtatt atggtgtaat gcacaattta
     6481 gaaaactccc atgcagttgc aataaaaata gtatggaaaa tcaaaacaat tgtttcattt
     6541 ctttcgaaag tggattttaa agagtaaaat tgtagcgagg aggatcaacg tctgtatttg
     6601 ctgagttaaa tgttaaacat taggaataaa atatagagtt gagcttgtcc tgtgcttagt
     6661 ctgttatctt gcttagaggg tcactggctt ctcagataac aaacagacct cgcctctgtt
     6721 ttattatttg gagtattgag cacagagtaa gctggacaaa aaactctggt tttctaaagg
     6781 tgaaacgtct tcacctttac tctctttgtt tgtttgtttg tctctgtttg ttgaaatgat
     6841 tgttagaagt ttcttttatt tttcaggcgg atttacttaa gtattaacta gcaagacata
     6901 agcaaatacc ttagtttaat ttgtttatac atatttaaag cataaacaaa attgaatatt
     6961 ttctcactgt tctgttatct gtatctttcc tgaactagat agctttcttt caaacagtcc
     7021 tgcactccaa actatatatc tgatattgta ggaaaattat ttaattaatt gaaaggctcc
     7081 caaaagatga ggcattttat aatggttcta ttaagtgttt gggatgtctt cactaattgt
     7141 tccactaaag tgctagtgag cttttggtac ataagtatat actatttgtg ttctatttta
     7201 ggagtgtata ttactccaca gttttctgta cagtcctgct attttatatt ttgattttat
     7261 aaacacacac acacacacac acacacacac acacaccaca ccacaccaca ccacaccaca
     7321 ccacaccaca ccacaccaca ccacaccaac ttaacttctg gtaagtctac ttaataaata
     7381 gtttggcatt tcccttccca tgagaaatga ctgacagtct tgatttttat ggaatagcat
     7441 tttggcagtt gtattgtctc tactgtgatt ctgattgctg tcattgttcc ttctctgatt
     7501 cggataccac attggatgcc tacctccctt gcaagcatgt tcctttaatc tccttctcgt
     7561 tggtggctta agggttccag aaaactgacc ttatttagta agctgctcca gaaaaacgtg
     7621 agtaatctaa tagacaggaa caattagcca tagtaataac gttgccaacg aggccccatc
     7681 aagctgtgac agtagcagta agggatattt caacattcaa aaaaattaat gtaatttaaa
     7741 ataatgtttt ctaccagcct ttatcacaca cagaaataca ttaaagctgg tcccaacttt
     7801 accccaaatg tagttgtctc tttccaagtg aatcagagca gttattttgg aatcaagctg
     7861 ttgcttccta gcctggattt ttctcaccat tgactttagg agacacaata ctgatagcag
     7921 aatccttcag cctcccagca caggaaacca taaagtgaac attcagatct gatctgatct
     7981 attaagcagc tcagtttcac ctgtaattaa aattagacat ctccatgcaa actaaaaact
     8041 gttgctagag tcaacagaag aaccaagggt ggtgtttcta gaatcacaac atttaaaatg
     8101 ggaagcaaaa gaatctagac tatgggtttc attagaggta ggcgtgaagg atgaaggctc
     8161 tgcttcactc tttttctttt tcattttgtg tgtgtttaaa atttcactct aaaggcgtag
     8221 ggattctttc agtcagtcag ggagtaactc acatgccacc atcttttatt caaaggcagg
     8281 cacagatgtt ccttctgcag ggaaggtttt aatttatgaa aatgatattg tttatgcatg
     8341 aatgaactgc atgcaccctg cataacgcat agcacttcca tctgatcaga gaaataccac
     8401 caaaccagcg ccaatgtcag aaacatattc tacacattta ctcactcaaa tgtttaatga
     8461 atatttggaa tgcattttca aaccataaga aatcttttaa attatctcct tcatttcttt
     8521 tatgaattgt taagcacatt ttaaagatac ttttacagtc tttatgtttg cttaatcctt
     8581 atgtgaagct aataaaaaac agtttttttt tttttgtgaa aaccaagaaa agaggggggg
     8641 tgagctgata gctttggacc aagaaaaata tttcatgtgg tttgcctatg tggtgcttga
     8701 tataatattt gggttctata ttcttgttct tcgtgagcaa ttgatgttcc tatcctagaa
     8761 aatacagctt tgtaatatta aaacacctgg aaaacttatt tctagaagaa gctggcagat
     8821 tatttcatag taggagagga attttagtta aatattttaa ctactgtata attcttatag
     8881 aataggacta ctccataaaa tttaagtttt atttttatca atttcaatgt tatatctata
     8941 tctattttta actattaaaa agaccatttc atgaacctac taatggaaca ccacatattc
     9001 tgagtagcct acagattttt agatttataa tatgttatag caaaatgata ttgcagccaa
     9061 attctaagta gtgcagttta ttgtttaaaa atatcagtat gttttcatca tttgaacatt
     9121 tatataaatt cttacaaaac tgatgcatct tctgcaagag gcctaaattc tgcaattact
     9181 aaaatgatat atggatcatt agctaacagt atgagccaag ctgttttagt tctataatta
     9241 tatgagaaat ttgaactgaa atatctatta aacaattaat aaaactaggt ttcaaatgca
     9301 gattggtttt gtcctaatgg aaaattacca agctatttat gaagttgttc ttaaaacccc
     9361 tctttcaaaa aagcatctat agcctcttat tttatgaaag aaaaaatacc taaataaaaa
     9421 tttcagttca agtaaccatg aaaaactaaa agagccaatg tgacatcttg tggaacccca
     9481 ggactgaaga ttcatgttat cattgtaaga ggcactctgg agtcagatga gcagtgattt
     9541 gtatggtggc ataatcattg gctcctggcc aggcattgtg tttcaatgga cgccattatc
     9601 aggaaccatt cagctagaaa tctgcactgt atgcacgcag aagttgaaat cgattttcga
     9661 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
     9721 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
     9781 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattcgaa
     9841 cacgcagatg cagtcggggc ggcgcggtcc caggtccact tcgcatatta aggtgacgcg
     9901 tgtggcctcg aacaccgagc gaccctgcag gtcctcgcca tggatcctga tgatgttgtt
     9961 gattcttcta aatcttttgt gatggaaaac ttttcttcgt accacgggac taaacctggt
    10021 tatgtagatt ccattcaaaa aggtatacaa aagccaaaat ctggtacaca aggaaattat
    10081 gacgatgatt ggaaagggtt ttatagtacc gacaataaat acgacgctgc gggatactct
    10141 gtagataatg aaaacccgct ctctggaaaa gctggaggcg tggtcaaagt gacgtatcca
    10201 ggactgacga aggttctcgc actaaaagtg gataatgccg aaactattaa gaaagagtta
    10261 ggtttaagtc tcactgaacc gttgatggag caagtcggaa cggaagagtt tatcaaaagg
    10321 ttcggtgatg gtgcttcgcg tgtagtgctc agccttccct tcgctgaggg gagttctagc
    10381 gttgaatata ttaataactg ggaacaggcg aaagcgttaa gcgtagaact tgagattaat
    10441 tttgaaaccc gtggaaaacg tggccaagat gcgatgtatg agtatatggc tcaagcctgt
    10501 gcaggaaatc gtgtcaggcg atctctttgt gaaggaacct tacttctgtg gtgtgacata
    10561 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    10621 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    10681 tgatgaatgg gagcagtggt ggaatgcaga tccactagag tcgatcgacc tgcaggcatg
    10741 caagctgggt gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    10801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10861 acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    12001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    12061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    12121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    12181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    12241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    12301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    12361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
