LOCUS       sequence-60624-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-ATTC
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(590..608)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(591..608)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(626..642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(626..642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(639..661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    650..666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(674..680,681..698,699..704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    719..740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(857..874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat tcgcccttgc
       61 cgtcctagcg cgtctcgctt aaccccagag caggtcgtgg caatcgcctc caacattggc
      121 gggaaacagg cactcgagac tgtccagcgc ctgcttcccg tgctgtgcca agcgcacgga
      181 ctcaccccag agcaggtcgt ggccattgcc tcgaatggag ggggcaaaca ggcgttggaa
      241 accgtacaac gattgctgcc ggtgctgtgc caagcgcacg gcctcacccc agagcaggtc
      301 gtggccattg cctcgaatgg agggggcaaa caggcgttgg aaaccgtaca acgattgctg
      361 ccggtgctgt gccaagcgca cggattaacc ccagagcagg tcgtggcgat cgcaagccac
      421 gacggaggaa agcaagcctt ggaaacagta cagaggctgt tgcctgtgct gtgccaagcg
      481 cacgggctca gagacgtggg cacaggaaag aagggcgaat tccagcacac tggcggccgt
      541 tactagtgga tccgagctcg gtaccaagct tgatgcatag cttgagtatt ctatagtgtc
      601 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
//
