LOCUS       sequence-60625-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-ATTG
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(591..609)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(592..609)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(627..643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(627..643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(640..662)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    651..667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(675..681,682..699,700..705))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    720..741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa ttcgcccttg
       61 ccgtcctagc gcgtctcgct taaccccaga gcaggtcgtg gcaatcgcct ccaacattgg
      121 cgggaaacag gcactcgaga ctgtccagcg cctgcttccc gtgctgtgcc aagcgcacgg
      181 actcacccca gagcaggtcg tggccattgc ctcgaatgga gggggcaaac aggcgttgga
      241 aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac ggcctcaccc cagagcaggt
      301 cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct
      361 gccggtgctg tgccaagcgc acggattaac cccagagcag gtcgtggcaa tcgcgagcaa
      421 taacggcgga aaacaggctt tggaaacggt gcagaggctc cttccagtgc tgtgccaagc
      481 gcacgggctc agagacgtgg gcacaggaaa gaagggcgaa ttccagcaca ctggcggccg
      541 ttactagtgg atccgagctc ggtaccaagc ttgatgcata gcttgagtat tctatagtgt
      601 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      661 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      721 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      781 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
//
