LOCUS       sequence-60630-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-CAAC
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(589..607)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(590..607)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(625..641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(625..641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(638..660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    649..665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(673..679,680..697,698..703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    718..739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(856..873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt cgcccttgcc
       61 gtcctagcgc gtctcgctta accccagagc aggtcgtggc gatcgcaagc cacgacggag
      121 gaaagcaagc cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacggac
      181 tcaccccaga gcaggtcgtg gcaatcgcct ccaacattgg cgggaaacag gcactcgaga
      241 ctgtccagcg cctgcttccc gtgctgtgcc aagcgcacgg cctcacccca gagcaggtcg
      301 tggcaatcgc ctccaacatt ggcgggaaac aggcactcga gactgtccag cgcctgcttc
      361 ccgtgctgtg ccaagcgcac ggattaaccc cagagcaggt cgtggcgatc gcaagccacg
      421 acggaggaaa gcaagccttg gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc
      481 acgggctcag agacgtgggc acaggaaaga agggcgaatt ccagcacact ggcggccgtt
      541 actagtggat ccgagctcgg taccaagctt gatgcatagc ttgagtattc tatagtgtca
      601 cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      661 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      721 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      781 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      841 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
//
