LOCUS       sequence-60651-e        4800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Bonnet J, Yin P, Ortiz ME, Subsoontorn P, Endy D
  TITLE     Amplifying genetic logic gates.
  JOURNAL   Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758.
            Epub 2013 Mar 28.
  PUBMED    23539178
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232758"; journalName: "Science"; date: "2013-05-3-
            3"; volume: "340"; issue: "6132"; pages: "599-603"
COMMENT     Sequence Label: OR gate
FEATURES             Location/Qualifiers
     source          1..4800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    129..178
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     terminator      187..258
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      274..301
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    312..359
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             448..1161
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1184..1255
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1271..1298
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1305..1325
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1361..1392
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      1517..1611
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2103..2325
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2373..3323
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             join(3612..3680,3681..4472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      complement(4602..4631)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(4725..4754)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaaaaaatt tatttgcttt cgcatctttt tgtacctata
       61 atgtgtggaa agcttatgcc aacacaatta acatctcaat caaggtaaat gctttttgct
      121 ttttttgctc ggccggcttg tcgacgacgg cggtctccgt cgtcaggatc atccgggccc
      181 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      241 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      301 gtttatatcg tggtttgtct ggtcaaccac cgcggtctca gtggtgtacg gtacaaaccc
      361 gcgagttttt atttcgttta tttcaattaa ggtaactaaa aaactccttt tactagagaa
      421 aagatctttt aagaaggaga tatacatatg cgtaaaggcg aagagctgtt cactggtgtc
      481 gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc
      541 gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt
      601 aaactgccgg taccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt
      661 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      721 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      781 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat cgactttaaa
      841 gaagacggca atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac
      901 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
      961 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccgat cggtgatggt
     1021 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
     1081 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
     1141 ggtatggatg aactgtacaa atgatgatac tagagccagg catcaaataa aacgaaaggc
     1201 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag tagcggccgc
     1321 tgcaggagtc actaagggtt agttagttag attagcagaa agtcaaaagc ctccgaccgg
     1381 aggcttttga ctaaaacttc ccttggggtt atcattgggg ctcactcaaa ggcggtaatc
     1441 agataaaaaa aatccttagc tttcgctaag gatgatttct gctagagctg tcagaccaag
     1501 tttacgagct cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
     1561 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagcact
     1621 agggacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc
     1681 agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag
     1741 gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc
     1801 tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa
     1861 aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg
     1921 aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca
     1981 gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt
     2041 gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta
     2101 gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc
     2161 tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta
     2221 cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac
     2281 ccacaactca aaggaaaagg acatgtaatt atcattgact agcccatctc aattggtata
     2341 gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa
     2401 atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct
     2461 gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt
     2521 tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat
     2581 tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta
     2641 aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat
     2701 tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata
     2761 atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat
     2821 taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat
     2881 ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg
     2941 ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata
     3001 agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc
     3061 tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca
     3121 ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg
     3181 caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg
     3241 atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac
     3301 tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca
     3361 agactaacaa acaaaagtag aacaactgtt caccgttaga tatcaaaggg aaaactgtcc
     3421 atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt
     3481 ggtgcattta aagctgttca ccatgaacag atcgacaatg taactactag agctaaatac
     3541 attcaaatat ctatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3601 aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3661 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gccgaagatc
     3721 agttgggtgc acgtgtgggt tacatcgaac tggacctcaa cagcggtaag attcttgaga
     3781 gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctctgtggcg
     3841 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3901 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggac ggcatgacag
     3961 tacgcgaatt atgcagcgct gccataacca tgagtgataa cacggcggcc aacttacttc
     4021 tgacaacgat cggaggaccg aaggagctta ccgctttttt gcacaacatg ggtgatcatg
     4081 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4141 acaccacgat gcctgtagct atggcaacaa cgttgcgcaa actcttaact ggcgaacttc
     4201 ttactctcgc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4261 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4321 agcgtgggtc ccgcggtatt attgcagccc tggggccaga tggtaagccc tcccgtatcg
     4381 tagttatcta cacgacgggg agccaggcaa ctatggacga acgtaatcgc cagatcgctg
     4441 agataggtgc ctccctgatt aagcattggt aataatacta gctccggcaa aaaaacgggc
     4501 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgtttgccac
     4561 ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg ccgaagaaag gcccacccgt
     4621 gaaggtgagc cagtgagttg attgctacgt aattagttag ttagccctta gtgactctgc
     4681 cacctgacgt ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc
     4741 cgtgaaggtg agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc
//
