LOCUS       sequence-60666-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ATTYA
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgtggt
      121 ctgtgaggcc ctcagacatg gccaagaaaa ctttcaaccc catccgagcc attgtggaca
      181 acatgaaggt gaaaccaaat ccaaacaaaa ccatgatttc cctgtccatt ggggacccta
      241 ctgtgtttgg aaacctgcct acagaccctg aagttaccca ggcaatgaaa gatgccctgg
      301 actcgggcaa atataatggc tatgccccat ccatcggctt cctatccagt cgggaggaga
      361 ttgcttctta ttaccactgt cctgaggcac ccctagaagc taaggacgtc attctgacaa
      421 gtggctgcag ccaagctatt gacctttgtt tagctgtgtt ggccaaccca gggcaaaaca
      481 tcctggttcc aagacctggt ttctctctct acaagactct ggctgagtct atgggaattg
      541 aggtcaaact ctacaatttg ttgccagaga aatcttggga aattgacctg aaacaactgg
      601 aatatctaat tgatgaaaag acagcttgtc tcattgtcaa taatccatca aacccctgtg
      661 ggtcagtgtt cagcaaacgt catcttcaga agattctggc agtggctgca cggcagtgtg
      721 tccccatctt agctgatgag atctatggag acatggtgtt ttcggattgc aaatatgaac
      781 cactggccac cctcagcacc gatgtcccca tcctgtcctg tggagggctg gccaagcgct
      841 ggctggttcc tggctggagg ttgggctgga tcctcattca tgaccgaaga gacatttttg
      901 gcaatgagat ccgagatggg ctg
//
