LOCUS       sequence-60667-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SERCA
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgctgc
      121 cgcactcagt gttgttagag atacaaaagg aattattaga ctacaaagga gttggcatta
      181 gtgttcttga aatgagtcac aggtcatcag attttgccaa gattattaac aatacagaga
      241 atcttgtgcg ggaattgcta gctgttccag acaactataa ggtgattttt ctgcaaggag
      301 gtgggtgcgg ccagttcagt gctgtcccct taaacctcat tggcttgaaa gcaggaaggt
      361 gtgcggacta tgtggtgaca ggagcttggt cagctaaggc cgcagaagaa gccaagaagt
      421 ttgggactat aaatatcgtt caccctaaac ttgggagtta tacaaaaatt ccagatccaa
      481 gcacctggaa cctcaaccca gatgcctcct acgtgtatta ttgcgcaaat gagacggtgc
      541 atggtgtgga gtttgacttt atacccgatg tcaagggagc agtactggtt tgtgacatgt
      601 cctcaaactt cctgtccaag ccagtggatg tttccaagtt tggtgtgatt tttgctggtg
      661 cccagaagaa tgttggctct gctggggtca ccgtggtgat tgtccgtgat gacctgctgg
      721 ggtttgccct ccgagagtgc ccctcggtcc tggaatacaa ggtgcaggct ggaaacagct
      781 ccttgtacaa cacgcctcca tgtttcagca tctacgtcat gggcttggtt ctggagtgga
      841 ttaaaaacaa tggaggtgcc gcggccatgg agaagcttag ctccatcaaa tctcaaacaa
      901 tttatgagat
//
