LOCUS       sequence-60669-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCYT2A
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggggg
      121 gcaggcgcgc cgtgagggtg tggtgcgatg gctgctatga catggtgcat tacggccact
      181 ccaaccagct gcgccaggca cgggccatgg gtgactacct catcgtaggc gtgcacaccg
      241 atgaggagat cgccaagcac aaggggcccc cggtgttcac tcaggaggag agatacaaga
      301 tggtgcaggc catcaaatgg gtggacgagg tggtgccagc ggctccctac gtcactacac
      361 tagagaccct ggacaaatac aactgtgact tctgtgttca cggcaatgac atcaccctga
      421 ctgtagatgg ccgggacacc tatgaggaag taaagcaggc tgggaggtac agagaatgca
      481 agcgcacgca aggggtgtcc accacagacc tcgtgggccg catgctgctg gtaaccaaag
      541 cccatcacag cagccaggag atgtcctctg agtaccggga gtatgcagac agttttggca
      601 agtgccctgg tgggcggaac ccctggaccg gggtatccca gttcctgcag acatctcaga
      661 agatcatcca gtttgcttct gggaaggagc cccagccagg ggagacagtc atctatgtgg
      721 ctggtgcctt cgacctgttc cacatcgggc atgtggactt cctggagaag gtgcacaggc
      781 tggcagagag gccctacatc atcgcgggct tacactttga ccaggaggtc aatcactaca
      841 aggggaagaa ctaccccatc atgaatctgc atgaacggac tctgagcgtg ctggcctgcc
      901 ggtacgtgtc agaagtggtg attggagccc cgtacgcggt
//
