LOCUS       sequence-60677-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CTPS2A
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgaaaa
      121 tatgctccat agccctggtt ggcaaataca ccaagctcag agactgctac gcctctgtgt
      181 tcaaagccct ggaacactca gccctggcca tcaaccacaa gttgaatctg atgtacatag
      241 actccattga tctggagaag atcactgaaa ccgaggaccc tgtgaaattt catgaagctt
      301 ggcagaagct atgcaaagct gatggtattc ttgtgcctgg aggctttgga atcagaggaa
      361 cattgggaaa actccaggcg atttcttggg caaggacaaa gaagattcct tttctgggag
      421 tttgtcttgg gatgcaacta gcagtgatag agtttgcaag aaactgcctt aacttgaaag
      481 atgctgattc cacagagttt aggccaaatg ccccagttcc tctggtgatt gatatgcccg
      541 agcacaaccc tggcaatttg ggaggaacaa tgagactggg aataagaaga actgttttca
      601 aaactgaaaa ttcaatatta aggaaacttt atggtgatgt tccttttata gaagaaagac
      661 acagacatcg gttcgaggta aaccctaacc tgatcaaaca atttgagcag aatgacttaa
      721 gttttgtagg tcaggatgtt gatggagaca ggatggaaat cattgaactg gcaaatcatc
      781 cttattttgt tggtgtccag ttccatcctg agttttcttc taggccgatg aagccttccc
      841 ctccgtatct ggggctgtta cttgcagcaa ctgggaacct gaatgcctac ttgcaacagg
      901 gttgca
//
