LOCUS       sequence-60678-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SKILA
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             488..505
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(558..576)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      572..619
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(743..762)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgcctt
      121 cagatagctc cacagaactc actcagactg tgttggaagg ggaatctatt tcttgttttc
      181 aagttggagg agaaaagaga ctctgtttgc cccaagtctt aaattctgtt ctccgagaat
      241 ttacactcca gcaaataaat acagtgtgtg atgaactgta catatattgt tcaaggtgta
      301 cttcagacca gcttcatatc ttaaaggtac tgggcatact tccattcaat gccccatcct
      361 gtgggctgat tacattaact gatgcacaaa gattatgtaa tgctttattg cggccacgaa
      421 cttgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      481 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      541 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      601 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      661 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      721 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      781 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      841 ttacggcacc tcgaccccaa aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      901 gccctgatag acggtt
//
