LOCUS       sequence-60679-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX19A
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggagg
      121 acagagctgc ccagtcctta ctcaacaagc tgatcagaag caaccttgtt gataacacaa
      181 accaagtgga agtcctgcag cgggatccaa actcccctct gtactcggtg aagtcttttg
      241 aagagcttcg gctgaaacca cagcttctcc aaggagtcta tgccatgggt ttcaatcgtc
      301 catccaagat acaagagaac gcattgccac tgatgcttgc tgagccccca cagaacttaa
      361 ttgcccaatc tcagtctggt actggtaaaa cagctgcctt cgtgctggcc atgcttagcc
      421 aagtagaacc tgcaaacaaa tacccccagt gtctatgtct ctccccaacg tatgagctcg
      481 ccctccaaac aggaaaagtg attgaacaaa tgggcaaatt ttaccctgaa ctgaagctag
      541 cttatgctgt tcgaggcaat aaattggaaa gaggccagaa gatcagtgag cagattgtca
      601 ttggcacccc tgggactgtg ctggactggt gctccaagct caagttcatt gatcccaaga
      661 aaatcaaggt gtttgttctg gatgaggctg atgtcatgat agccactcag ggccaccaag
      721 atcagagcat ccgcatccag aggatgctgc ccaggaactg ccagatgctg cttttctccg
      781 ccacctttga agactctgtg tggaagtttg cccagaaagt ggtcccagac ccaaacgtta
      841 tcaaactgaa gcgtgaggaa gagaccctgg acaccatcaa gcagtactat gtcctgtgca
      901 gcagcagaga cgagaagttc caggccttgt gtaacctcta cg
//
